6O3W
Crystal structure of yeast Nrd1 CID in complex with Sen1 NIM1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-27 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 103.418, 109.103, 86.743 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.179 - 2.100 |
R-factor | 0.1864 |
Rwork | 0.185 |
R-free | 0.20410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3clj |
RMSD bond length | 0.006 |
RMSD bond angle | 0.720 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.084 | 0.679 |
Number of reflections | 27909 | |
<I/σ(I)> | 16.8 | |
Completeness [%] | 96.2 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | sodium citrate, lithium chloride, PEG 6000 |