6O3N

Cross-alpha Amyloid-like Structure alphaAmA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.11583
Spacegroup name	I 41 2 2
Unit cell lengths	164.816, 164.816, 164.134
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	116.500 - 3.700
R-factor	0.29888
Rwork	0.297
R-free	0.31925
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6c4z
RMSD bond length	0.008
RMSD bond angle	1.681
Data reduction software	XDS (VERSION Jan 26, 2018 BUILT=20180409)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	116.500	3.930
High resolution limit [Å]	3.700	3.700
Rmeas	0.098	2.110
Number of reflections	12514	1946
<I/σ(I)>	18.48	1.59
Completeness [%]	99.7	98
Redundancy	25.6	25.5
CC(1/2)	0.997	0.465

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	45% MPD, 0.25M NaH2PO4