6O3M

Unmodified tRNA(Pro) bound to Thermus thermophilus 70S (cognate)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	209.970, 450.710, 619.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.820 - 3.970
R-factor	0.2293
Rwork	0.228
R-free	0.25470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v5c
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.14_3260)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.820	49.820	4.070
High resolution limit [Å]	3.970	17.750	3.970
Rmerge	0.330	0.057	2.780
Rmeas	0.342	0.059	2.932
Total number of observations	6976690
Number of reflections	496012	5591	34930
<I/σ(I)>	6.32	33.26	0.96
Completeness [%]	98.6	92	94.7
Redundancy	14.066	14.776	9.375
CC(1/2)	0.999	0.999	0.293

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	12% MPD 0.1M, Arginine-HCl, 0.1M Tris-HCl pH7.6, 2.9% PEG 20K, 0.0006M BME