6O3E

mouse aE-catenin 82-883

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-12-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 32
Unit cell lengths	145.262, 145.262, 136.269
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.971 - 4.001
R-factor	0.215
Rwork	0.213
R-free	0.24290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4igg
Data reduction software	XDS
Data scaling software	Aimless (0.1.29)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.971	19.970	4.280
High resolution limit [Å]	4.000	11.310	4.000
Rmerge	0.080	0.029	1.240
Rmeas	0.095	0.035	1.469
Rpim	0.050	0.018	0.775
Number of reflections	26524	962	4874
<I/σ(I)>	7.6
Completeness [%]	97.6	80.4	98.8
Redundancy	3.5	3.2	3.5
CC(1/2)	0.998	0.998	0.563

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	50 mM Tris, pH 8.5, 60 mM lithium sulfate, 23.5 % PEG 400