6O3E
mouse aE-catenin 82-883
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 |
| Unit cell lengths | 145.262, 145.262, 136.269 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.971 - 4.001 |
| R-factor | 0.215 |
| Rwork | 0.213 |
| R-free | 0.24290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4igg |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.29) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.971 | 19.970 | 4.280 |
| High resolution limit [Å] | 4.000 | 11.310 | 4.000 |
| Rmerge | 0.080 | 0.029 | 1.240 |
| Rmeas | 0.095 | 0.035 | 1.469 |
| Rpim | 0.050 | 0.018 | 0.775 |
| Number of reflections | 26524 | 962 | 4874 |
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 97.6 | 80.4 | 98.8 |
| Redundancy | 3.5 | 3.2 | 3.5 |
| CC(1/2) | 0.998 | 0.998 | 0.563 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 50 mM Tris, pH 8.5, 60 mM lithium sulfate, 23.5 % PEG 400 |
