6O31

CRYSTAL STRUCTURE OF THE ACTIN-BINDING DOMAIN OF HUMAN ALPHA-ACTININ-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 41 21 2
Unit cell lengths	38.069, 38.069, 302.655
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.860 - 1.510
R-factor	0.21057
Rwork	0.208
R-free	0.25391
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r0o
RMSD bond length	0.013
RMSD bond angle	1.825
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.560
High resolution limit [Å]	1.510	1.510
Number of reflections	36924	3555
<I/σ(I)>	25.3	2.3
Completeness [%]	99.9	99.9
Redundancy	8.9	8.9
CC(1/2)	0.989	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	295	50mM Sodium Chloride, 5% Glycerol, 1mM EDTA, 15-18% PEG5000-MME