Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.750, 61.400, 91.090 |
| Unit cell angles | 90.00, 107.74, 90.00 |
Refinement procedure
| Resolution | 48.700 - 2.300 |
| R-factor | 0.22525 |
| Rwork | 0.223 |
| R-free | 0.26505 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wd6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.407 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.760 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.062 | 0.241 |
| Rmeas | 0.068 | 0.267 |
| Rpim | 0.027 | 0.113 |
| Number of reflections | 19718 | 1940 |
| <I/σ(I)> | 15 | |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 5.7 | 5.3 |
| CC(1/2) | 0.997 | 0.985 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 0.1M HEPES PH 7.5, 0.2M CALCIUM CHLORIDE, 9% PEG 3350, 5% ISOPROPYL ALCOHOL, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291.15K |
