6O19

Crystal Structure of Pho7 complex with pho1 promoter site 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97910
Spacegroup name	P 1 21 1
Unit cell lengths	53.169, 37.581, 57.564
Unit cell angles	90.00, 104.23, 90.00

Refinement procedure

Resolution	43.571 - 1.596
R-factor	0.1824
Rwork	0.181
R-free	0.20270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6e33
RMSD bond length	0.008
RMSD bond angle	1.048
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3211: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.596	1.596
Rpim	0.027	0.131
Number of reflections	28959	1429
<I/σ(I)>	29.7	0.979
Completeness [%]	97.9	95.1
Redundancy	4	3.5
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Tris.HCl pH 8.0, 0.2M NaOAc, 35% PEG 4000