6O19
Crystal Structure of Pho7 complex with pho1 promoter site 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.169, 37.581, 57.564 |
| Unit cell angles | 90.00, 104.23, 90.00 |
Refinement procedure
| Resolution | 43.571 - 1.596 |
| R-factor | 0.1824 |
| Rwork | 0.181 |
| R-free | 0.20270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6e33 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.048 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3211: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.596 | 1.596 |
| Rpim | 0.027 | 0.131 |
| Number of reflections | 28959 | 1429 |
| <I/σ(I)> | 29.7 | 0.979 |
| Completeness [%] | 97.9 | 95.1 |
| Redundancy | 4 | 3.5 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Tris.HCl pH 8.0, 0.2M NaOAc, 35% PEG 4000 |
