6O18
Unliganded alpha-L-fucosidase AlfC from Lactobacillus casei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.03320 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 89.298, 137.119, 264.709 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.110 - 2.550 |
| R-factor | 0.2096 |
| Rwork | 0.208 |
| R-free | 0.24220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pct |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.382 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.1) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.410 | 29.410 | 2.630 |
| High resolution limit [Å] | 2.550 | 10.510 | 2.550 |
| Rmerge | 0.063 | 0.037 | 1.256 |
| Rmeas | 0.069 | 0.040 | 1.387 |
| Rpim | 0.029 | 0.017 | 0.580 |
| Total number of observations | 291121 | ||
| Number of reflections | 53160 | 805 | 4566 |
| <I/σ(I)> | 12.7 | ||
| Completeness [%] | 99.7 | 95 | 100 |
| Redundancy | 5.5 | 5.1 | 5.5 |
| CC(1/2) | 0.999 | 0.998 | 0.557 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 250 nL 20 mg/ml wild-type AlfC + 250 nL mother liquor (18% w/v PEG3350, 0.1 M Bis-Tris propane, pH 7, 20 mM sodium/potassion phosphate, 1% v/v glycerol), crystals appeared after five days |
