6O17
Recombinant Human Insulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-17 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 77.633, 77.633, 77.633 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.710 - 1.580 |
| R-factor | 0.18346 |
| Rwork | 0.182 |
| R-free | 0.20112 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5viz |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.983 |
| Data reduction software | HKL-2000 (2.3.8) |
| Data scaling software | HKL-2000 (2.3.8) |
| Phasing software | CrystalClear (2.3.8) |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.043 | |
| Rpim | 0.421 | 0.421 |
| Number of reflections | 10867 | 541 |
| <I/σ(I)> | 67.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7.7 | |
| CC(1/2) | 0.646 | 0.646 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 298 | PEG 6000 30% |
