6NZS
Dextranase AoDex KQ11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2017-04-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 214.071, 55.099, 62.188 |
| Unit cell angles | 90.00, 92.45, 90.00 |
Refinement procedure
| Resolution | 43.596 - 1.400 |
| R-factor | 0.1683 |
| Rwork | 0.168 |
| R-free | 0.18250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wwg |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.824 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.410 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmerge | 0.052 | 0.420 |
| Number of reflections | 143197 | |
| <I/σ(I)> | 15.4 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | magnesium chloride, HEPES, PEG |
