6NZC
Crystal structure of E. coli fumarase C N326A variant with closed SS Loop at 1.40 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-13 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9787 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 103.819, 217.389, 86.109 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.420 - 1.403 |
| R-factor | 0.1369 |
| Rwork | 0.136 |
| R-free | 0.16130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fuo |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 108.700 | 1.430 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.114 | 1.270 |
| Number of reflections | 187757 | 9382 |
| <I/σ(I)> | 14.3 | 2.1 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 14.5 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 298 | PEG 3350, citrate |
