6NZ9
Crystal structure of E. coli fumarase C bound to citrate at 1.53 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-12 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0781 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 103.157, 216.679, 86.936 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.975 - 1.528 |
R-factor | 0.1573 |
Rwork | 0.157 |
R-free | 0.17340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fuo |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.553 | 1.550 |
High resolution limit [Å] | 1.528 | 1.528 |
Rmerge | 0.088 | 0.975 |
Number of reflections | 146755 | 7249 |
<I/σ(I)> | 14.7 | |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 10.9 | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 298 | PEG 3350, citrate |