6NZ9
Crystal structure of E. coli fumarase C bound to citrate at 1.53 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-12 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0781 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 103.157, 216.679, 86.936 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.975 - 1.528 |
| R-factor | 0.1573 |
| Rwork | 0.157 |
| R-free | 0.17340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fuo |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.553 | 1.550 |
| High resolution limit [Å] | 1.528 | 1.528 |
| Rmerge | 0.088 | 0.975 |
| Number of reflections | 146755 | 7249 |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 10.9 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 298 | PEG 3350, citrate |
