6NYR

The crystal structure of CroV588 a novel circular LRR protein structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	65.403, 172.620, 92.333
Unit cell angles	90.00, 94.79, 90.00

Refinement procedure

Resolution	49.090 - 2.431
R-factor	0.2222
Rwork	0.220
R-free	0.25680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.670
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.090	2.518
High resolution limit [Å]	2.431	2.431
Number of reflections	76163
<I/σ(I)>	11.59
Completeness [%]	99.6
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Mes pH 6.0, 20% PEG3000, 0.1M MgCl2