6NYQ
Crystal structure of glycosylated lysosomal membrane protein (GLMP) luminal domain bound to a Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.924, 55.238, 99.639 |
| Unit cell angles | 90.00, 99.35, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.850 |
| R-factor | 0.16581 |
| Rwork | 0.164 |
| R-free | 0.20411 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.677 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 71330 | |
| <I/σ(I)> | 9.9 | 1.97 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.611 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 8% tacsimate pH 6.0, 16% PEG 3350, 10 mM phenol |
