Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-23 |
| Detector | SBC-3 |
| Wavelength(s) | 0.98066 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 42.572, 101.141, 68.038 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.430 - 1.893 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.24780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rsy |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.14) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 26.430 | 1.930 |
| High resolution limit [Å] | 1.893 | 5.160 | 1.900 |
| Rmerge | 0.050 | 0.037 | 0.379 |
| Number of reflections | 10420 | 658 | 279 |
| <I/σ(I)> | 17 | ||
| Completeness [%] | 86.5 | 97.5 | 47.5 |
| Redundancy | 7.2 | 7.9 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 30% PEG-MME 2000, 0.1 M bis-tris propane pH 6.8, 0.1 M NaCl, 0.5 mM TCEP |
