6NXY
Crystal structure of Arabidopsis thaliana cytosolic triosephosphate isomerase C218D mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9787 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 38.993, 163.486, 97.744 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.872 - 1.050 |
| R-factor | 0.1659 |
| Rwork | 0.166 |
| R-free | 0.17110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4obt |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.870 | 1.070 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.079 | 0.496 |
| Rmeas | 0.086 | 0.548 |
| Rpim | 0.035 | 0.227 |
| Total number of observations | 874388 | |
| Number of reflections | 145559 | 7067 |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 100.0 | 99.5 |
| Redundancy | 6 | 5.7 |
| CC(1/2) | 0.997 | 0.835 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.2M Sodium acetate trihydrate 0.1M TRIS hydrochloride 8.5 30% w/v Polyethylene glycol 4,000 |
