6NXQ
Crystal structure of Arabidopsis thaliana cytosolic triosephosphate isomerase C13A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002+ |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-08-13 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5419 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 38.468, 162.683, 97.233 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.470 - 1.960 |
| R-factor | 0.1705 |
| Rwork | 0.169 |
| R-free | 0.19980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4obt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.360 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.470 | 2.010 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.088 | 0.434 |
| Rmeas | 0.096 | 0.480 |
| Rpim | 0.039 | 0.200 |
| Number of reflections | 22354 | 1502 |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 99.4 | 97.1 |
| Redundancy | 5.9 | 5.4 |
| CC(1/2) | 0.998 | 0.898 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.2 M Magnesium chloride hexahydrate, 0.1 M TRIS hydrochloride 8.5, 30 % w/v Polyethylene glycol 4,000 |
