6NXK

Ubiquitin binding variants

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	58.893, 63.368, 100.135
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.550 - 2.200
R-factor	0.22287
Rwork	0.221
R-free	0.26620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4s1z 4YII chain A
RMSD bond length	0.010
RMSD bond angle	1.340
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.550	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.069	0.583
Rpim	0.036	0.303
Number of reflections	17815
<I/σ(I)>	12
Completeness [%]	96.5	99
Redundancy	4.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.1M HEPES pH 7.7, 27.5% PEG3350, 2.5mM APC2 WHB (735-C) + 2.08mM Ubiquitin protein co-mixed