6NWX

Structure of mouse GILT, an enzyme involved in antigen processing

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.85
Spacegroup name	C 1 2 1
Unit cell lengths	170.440, 44.720, 78.170
Unit cell angles	90.00, 111.07, 90.00

Refinement procedure

Resolution	29.388 - 2.000
R-factor	0.1698
Rwork	0.168
R-free	0.20180
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.388	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.078
Rmeas	0.095
Number of reflections	37057	3685
<I/σ(I)>	8.1	1.05
Completeness [%]	99.2	99.43
Redundancy	3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	293	0.1 M HEPES 2.0% PEG400 2.0 M AmSO4