6NWO

Structures of the transcriptional regulator BgaR, a lactose sensor.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953723
Spacegroup name	I 1 2 1
Unit cell lengths	68.429, 40.482, 261.721
Unit cell angles	90.00, 90.33, 90.00

Refinement procedure

Resolution	43.600 - 2.110
R-factor	0.19559
Rwork	0.194
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nwh
RMSD bond length	0.008
RMSD bond angle	1.427
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.620	2.170
High resolution limit [Å]	2.110	2.110
Rmerge	0.103	0.672
Rpim	0.043	0.290
Number of reflections	41261	3104
<I/σ(I)>	9.4	2.7
Completeness [%]	98.7	91.7
Redundancy	6.7	6.3
CC(1/2)	0.997	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.7	293	5 mg/mL protein; 21.7% PEG 3350, 222mM MgCl2, 100mM bis-tris pH 5.7. Sitting drops were set up in 200 nL plus 200 nL volumes at 20C