6NWM
Structures of the transcriptional regulator BgaR, a lactose sensor.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953723 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 46.439, 87.528, 118.974 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.800 - 2.110 |
| R-factor | 0.20523 |
| Rwork | 0.204 |
| R-free | 0.23324 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6nwh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.474 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 1.990 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.165 | 1.776 |
| Rpim | 0.048 | 0.498 |
| Number of reflections | 36663 | 2389 |
| <I/σ(I)> | 9.4 | 1.8 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 13.1 | 13.5 |
| CC(1/2) | 0.998 | 0.761 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 5 mg/mL protein; 1.27 M sodium malonate, 100 mM HEPES pH 7.5. Sitting drops were set up in 200 nL plus 200 nL volumes with seeds at 20C |
