6NWM

Structures of the transcriptional regulator BgaR, a lactose sensor.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953723
Spacegroup name	P 2 21 21
Unit cell lengths	46.439, 87.528, 118.974
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.800 - 2.110
R-factor	0.20523
Rwork	0.204
R-free	0.23324
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nwh
RMSD bond length	0.008
RMSD bond angle	1.474
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.400	1.990
High resolution limit [Å]	1.940	1.940
Rmerge	0.165	1.776
Rpim	0.048	0.498
Number of reflections	36663	2389
<I/σ(I)>	9.4	1.8
Completeness [%]	99.8	99.4
Redundancy	13.1	13.5
CC(1/2)	0.998	0.761

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	5 mg/mL protein; 1.27 M sodium malonate, 100 mM HEPES pH 7.5. Sitting drops were set up in 200 nL plus 200 nL volumes with seeds at 20C