6NWJ
Structures of the transcriptional regulator BgaR, a lactose sensor.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953657 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 46.375, 87.010, 119.432 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.540 - 2.160 |
| R-factor | 0.18939 |
| Rwork | 0.188 |
| R-free | 0.21803 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6nwh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.484 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 2.220 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.208 | 1.167 |
| Rpim | 0.060 | 0.335 |
| Number of reflections | 26514 | 2098 |
| <I/σ(I)> | 11.6 | 3.5 |
| Completeness [%] | 99.0 | 92.6 |
| Redundancy | 13 | 12.7 |
| CC(1/2) | 0.996 | 0.827 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | 5 mg/mL protein; 1.25 M sodium malonate, 100 mM gly-gly pH 8.2 set up in 200 nL plus 200 nL sitting drops at 20C |
