6NWJ

Structures of the transcriptional regulator BgaR, a lactose sensor.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953657
Spacegroup name	P 2 21 21
Unit cell lengths	46.375, 87.010, 119.432
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.540 - 2.160
R-factor	0.18939
Rwork	0.188
R-free	0.21803
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nwh
RMSD bond length	0.008
RMSD bond angle	1.484
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.400	2.220
High resolution limit [Å]	2.160	2.160
Rmerge	0.208	1.167
Rpim	0.060	0.335
Number of reflections	26514	2098
<I/σ(I)>	11.6	3.5
Completeness [%]	99.0	92.6
Redundancy	13	12.7
CC(1/2)	0.996	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	293	5 mg/mL protein; 1.25 M sodium malonate, 100 mM gly-gly pH 8.2 set up in 200 nL plus 200 nL sitting drops at 20C