6NU7
Structure of sucrose-6-phosphate hydrolase from Lactobacillus gasseri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-21 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 103.149, 103.149, 101.978 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.023 - 1.900 |
| R-factor | 0.1676 |
| Rwork | 0.166 |
| R-free | 0.20340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pig |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.016 |
| Data reduction software | SAINT |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.940 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.287 | 0.961 |
| Rmeas | 0.297 | 1.024 |
| Rpim | 0.075 | 0.367 |
| Number of reflections | 44022 | 2755 |
| <I/σ(I)> | 13.7 | 3.4 |
| Completeness [%] | 99.9 | 98.5 |
| Redundancy | 31.6 | 15 |
| CC(1/2) | 0.988 | 0.719 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M buffer MMT pH 9.0; 25% (m/v) PEG 1500 |
