6NTX

Respiratory syncytial virus fusion protein N-terminal heptad repeat domain+VIQKI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-14
Detector	BRUKER SMART 6000
Wavelength(s)	1.5418
Spacegroup name	H 3 2
Unit cell lengths	51.967, 51.967, 299.092
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.760 - 2.200
R-factor	0.2367
Rwork	0.232
R-free	0.28460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kpe
RMSD bond length	0.015
RMSD bond angle	1.280
Data reduction software	PROTEUM2
Data scaling software	SADABS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.100	2.279
High resolution limit [Å]	2.200	2.200
Rmeas	0.130	1.645
Rpim	0.030	0.394
Number of reflections	8394	829
<I/σ(I)>	15.34	2.43
Completeness [%]	100.0	99.64
Redundancy	19.8	16.9
CC(1/2)	0.998	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	30 mM NaF, 30 mM NaBr, 30 mM NaI, 12.5% (v/v) 2-methyl-2,4-pentanediol, 12.5% (v/v) PEG 1000, 12.5% (w/v) PEG 3350 in 100 mM NaHEPES/MOPS buffer (pH 7.5)