6NTR
Crystal Structure of Beta-barrel-like Protein of Domain of Unknown Function DUF1849 from Brucella abortus
Replaces: 5UC2Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.360, 69.240, 83.239 |
| Unit cell angles | 90.09, 90.02, 78.66 |
Refinement procedure
| Resolution | 35.234 - 2.101 |
| R-factor | 0.2018 |
| Rwork | 0.195 |
| R-free | 0.24470 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.474 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.068 | 0.390 |
| Number of reflections | 57342 | |
| <I/σ(I)> | 19.96 | 1.47 |
| Completeness [%] | 94.9 | 68.1 |
| Redundancy | 2.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | MCSG2 F10: 24% w/v PEG1500, 20% w/v glycerol |
