6NTE
Crystal Structure of Synechocystis Dihydroxyacid Dehydratase (DHAD)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.790, 74.258, 80.680 |
Unit cell angles | 90.00, 90.15, 90.00 |
Refinement procedure
Resolution | 37.130 - 2.330 |
R-factor | 0.1752 |
Rwork | 0.172 |
R-free | 0.22490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gp4 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.988 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.13_2998) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.130 | 2.413 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.111 | 0.714 |
Rmeas | 0.124 | 0.805 |
Rpim | 0.056 | 0.368 |
Number of reflections | 40235 | 3829 |
<I/σ(I)> | 12.12 | 2.13 |
Completeness [%] | 99.2 | 95.25 |
Redundancy | 4.7 | 4.6 |
CC(1/2) | 0.997 | 0.709 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2M Magnesium chloride hexahydrate, 0.1M BIS-TRIS, 25% PEG 3,350 |