6NSV
Crystal structure of the human CHIP TPR domain in complex with a 5mer acetylated optimized peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1158 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 46.219, 71.920, 77.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.735 - 1.305 |
| R-factor | 0.2155 |
| Rwork | 0.214 |
| R-free | 0.24270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q49 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.120 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.730 | 1.352 |
| High resolution limit [Å] | 1.305 | 1.305 |
| Number of reflections | 60500 | |
| <I/σ(I)> | 11.09 | |
| Completeness [%] | 94.7 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.05 M CaCl2, 0.1 M HEPES pH 7, 28% PEG 4K, 0.01 M CoCl2 |
