6NSR
TetR family transcriptional regulator CifR C99T-C181R cysteine mutant complexed with 26bp double-strand operator DNA and apo-CifR C99T-C181R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-12 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 63.517, 165.569, 81.529 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 3.000 |
| R-factor | 0.2419 |
| Rwork | 0.239 |
| R-free | 0.26830 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.619 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.980 | 3.081 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 17781 | 5878 |
| <I/σ(I)> | 9.29 | |
| Completeness [%] | 99.6 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.998 | 0.793 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 0.1 M Tris, pH 7.5, 0.2 M magnesium chloride, 11% PEG1000, 11% PEG8000 |
