6NSN
TetR family transcriptional regulator CifR C99T-C181R Cysteines mutant complexed with 26bp double-strand operator DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-12 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.979075 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 63.968, 166.685, 82.979 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.861 - 2.600 |
R-factor | 0.2272 |
Rwork | 0.225 |
R-free | 0.26670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6nsr |
RMSD bond length | 0.010 |
RMSD bond angle | 1.109 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.861 | 2.693 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmeas | 0.111 | |
Number of reflections | 28028 | 2961 |
<I/σ(I)> | 17.41 | |
Completeness [%] | 99.9 | |
Redundancy | 12.2 | |
CC(1/2) | 0.999 | 0.723 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 0.1 M Tris, pH 7.5, 0.2 M magnesium chloride, 16% PEG4000 |