6NSM
TetR family transcriptional regulator CifR C99T-C107S-C181R Cysteines mutant complexed with 26bp double-strand operator DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979303 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 64.329, 167.897, 82.538 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.983 - 2.800 |
R-factor | 0.2286 |
Rwork | 0.227 |
R-free | 0.25550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6nsn |
RMSD bond length | 0.009 |
RMSD bond angle | 1.016 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.983 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmeas | 0.163 | |
Number of reflections | 22677 | 4159 |
<I/σ(I)> | 14.15 | |
Completeness [%] | 99.8 | |
Redundancy | 13.5 | |
CC(1/2) | 0.999 | 0.733 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 291 | 0.1 M Tris, pH 7.9, 0.2 M magnesium chloride, 18% PEG4000 |