6NSF
Crystal structure of the A/Brisbane/10/2007 (H3N2) influenza virus hemagglutinin G186V/L194P mutant in complex with 3'-SLNLN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | H 3 2 |
Unit cell lengths | 100.174, 100.174, 382.752 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
R-factor | 0.18355 |
Rwork | 0.182 |
R-free | 0.21361 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6aoq |
RMSD bond length | 0.010 |
RMSD bond angle | 1.481 |
Data reduction software | HKL-2000 (712) |
Data scaling software | HKL-2000 (712) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rpim | 0.030 | 0.210 |
Number of reflections | 44480 | |
<I/σ(I)> | 20.6 | 2.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 19.2 | 19.9 |
CC(1/2) | 1.000 | 0.890 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M CAPS pH 10.5 and 29% PEG 400 |