6NS9
Crystal structure of the IVR-165 (H3N2) influenza virus hemagglutinin apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | H 3 2 |
Unit cell lengths | 100.400, 100.400, 382.058 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.950 |
R-factor | 0.16792 |
Rwork | 0.167 |
R-free | 0.19319 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o5n |
RMSD bond length | 0.010 |
RMSD bond angle | 1.487 |
Data scaling software | HKL-2000 (712) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.010 |
High resolution limit [Å] | 1.950 | 1.950 |
Rpim | 0.040 | 0.220 |
Number of reflections | 54947 | 4957 |
<I/σ(I)> | 26 | 2.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 18.1 | 17.4 |
CC(1/2) | 1.000 | 0.920 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 44% 2-methyl-2,4-pentanediol, 0.1 M HEPES pH 7.0 |