6NRX

Crystal structure of DIP-eta IG1 homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	120
Detector technology	PIXEL
Collection date	2017-11-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	88.426, 67.133, 61.012
Unit cell angles	90.00, 128.82, 90.00

Refinement procedure

Resolution	48.080 - 1.900
R-factor	0.2339
Rwork	0.232
R-free	0.26930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eo9
RMSD bond length	0.003
RMSD bond angle	0.636
Data reduction software	XDS (Nov 11, 2017)
Data scaling software	XDS (Nov 11, 2017)
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.13rc2_2986)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.041	0.559
Rmeas	0.049	0.668
Number of reflections	21928	1506
<I/σ(I)>	12.33	1.99
Completeness [%]	98.3	93.3
Redundancy	3.4	3.32
CC(1/2)	0.998	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	294	0.1 M sodium citrate, pH 5.5, 45% (w/v) PEG 200