6NRE
Monomeric Lipocalin Can F 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 67.154, 67.154, 72.488 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.160 - 2.060 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.672 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.490 | 2.110 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.051 | 0.412 |
| Rmeas | 0.052 | 0.457 |
| Rpim | 0.012 | 0.194 |
| Total number of observations | 69256 | 5492 |
| Number of reflections | 12122 | 872 |
| <I/σ(I)> | 38.8 | 0.1 |
| Completeness [%] | 99.4 | 93 |
| Redundancy | 17 | 4.9 |
| CC(1/2) | 0.985 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 2-propanol, sodium acetate, polyethylene glycol ether 550 |
