6NR5

Human LSD1 in complex with Phenelzine sulfate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 61 2 2
Unit cell lengths	189.361, 189.361, 109.031
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.916 - 2.900
R-factor	0.2223
Rwork	0.220
R-free	0.27320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.643
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.920	3.080
High resolution limit [Å]	2.900	2.900
Number of reflections	26000	4134
<I/σ(I)>	11
Completeness [%]	99.9
Redundancy	20.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	PEG 3350 95, 0.1M Ammonium citrate pH6.5