6NQQ

Crystal structure of fast switching M159T mutant of fluorescent protein Dronpa (Dronpa2), Y63(2,3,5-F3Y)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.195
Spacegroup name	P 1 21 1
Unit cell lengths	80.837, 86.375, 143.590
Unit cell angles	90.00, 95.06, 90.00

Refinement procedure

Resolution	39.520 - 2.600
R-factor	0.265
Rwork	0.263
R-free	0.31100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uts
RMSD bond length	0.008
RMSD bond angle	1.392
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13RC2_2986: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.524	2.670
High resolution limit [Å]	2.600	2.600
Rmeas	0.120	1.110
Number of reflections	59806	4354
<I/σ(I)>	10.6	1.7
Completeness [%]	98.5	97.9
Redundancy	7	6.8
CC(1/2)	0.999	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	0.1 M Tris-HCl at pH 7.4, 0.1 M MgCl2, 18% PEG 3350