6NQP

Crystal structure of fast switching M159T mutant of fluorescent protein Dronpa (Dronpa2), Y63(2,3-F2Y)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.195
Spacegroup name	P 1 21 1
Unit cell lengths	79.300, 85.970, 143.020
Unit cell angles	90.00, 94.93, 90.00

Refinement procedure

Resolution	39.250 - 2.200
R-factor	0.227
Rwork	0.225
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uts
RMSD bond length	0.007
RMSD bond angle	1.064
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13RC2_2986: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.245	2.260
High resolution limit [Å]	2.200	2.200
Rmeas	0.099	1.200
Number of reflections	94353	6792
<I/σ(I)>	15.1	1.7
Completeness [%]	97.0	95.3
Redundancy	11.2	7.8
CC(1/2)	0.999	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris-HCl at pH 7.4, 0.1 M MgCl2, 16% PEG 3350