6NQL
Crystal structure of fast switching M159T mutant of fluorescent protein Dronpa (Dronpa2), Y63(3-ClY)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.127 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.705, 86.280, 143.349 |
| Unit cell angles | 90.00, 95.08, 90.00 |
Refinement procedure
| Resolution | 39.590 - 2.147 |
| R-factor | 0.198 |
| Rwork | 0.197 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uts |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.137 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13RC2_2986: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.590 | 2.210 |
| High resolution limit [Å] | 2.147 | 2.147 |
| Rmeas | 0.190 | 1.430 |
| Number of reflections | 107363 | 7923 |
| <I/σ(I)> | 9.2 | 1.5 |
| Completeness [%] | 99.0 | 98.6 |
| Redundancy | 8.2 | 8.3 |
| CC(1/2) | 0.997 | 0.774 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 0.1 M Tris-HCl at pH 7.4, 0.1 M MgCl2, 12% PEG 3350 |
