6NQ8
Crystal structure of YetJ mutant from Bacillus Subtilis - D171E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 218.090, 117.296, 52.407 |
| Unit cell angles | 90.00, 92.97, 90.00 |
Refinement procedure
| Resolution | 39.082 - 3.100 |
| R-factor | 0.2446 |
| Rwork | 0.243 |
| R-free | 0.27050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pgs |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.454 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.150 | 3.310 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 76248 | |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 99.1 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1 M MOPS, pH 7.5, 38% PEG 400, and 0.2 M NaCl |
