6NPW
SSu72/Sympk in complex with Ser2/Ser5 phosphorylated peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-17 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.033 |
Spacegroup name | P 4 |
Unit cell lengths | 127.702, 127.702, 105.979 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.943 - 2.486 |
R-factor | 0.1908 |
Rwork | 0.189 |
R-free | 0.23490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4imi |
RMSD bond length | 0.005 |
RMSD bond angle | 0.694 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.550 |
High resolution limit [Å] | 2.486 | 2.490 |
Number of reflections | 59967 | 4110 |
<I/σ(I)> | 11.97 | |
Completeness [%] | 100.0 | |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 12% PEG3350 (w/v) and 100 mM HEPE pH 8.0 |