6NP6

Crystal structure of the sensor domain of the transcriptional regulator HcpR from Porphyromonas Gingivalis

Replaces:  5V3E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-18
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.18076
Spacegroup name	C 2 2 21
Unit cell lengths	133.468, 138.851, 44.546
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.729 - 2.600
R-factor	0.2671
Rwork	0.264
R-free	0.29420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SV30
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_3092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.730	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.066	0.264
Number of reflections	13182	1848
<I/σ(I)>	11.62
Completeness [%]	99.8	99.4
Redundancy	7.3	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	0.1M Na Acetate (pH 4.5), 1-2-1.4M Ammonium sulfate, 0.2M NaCl