6NOU

An scFv derived from ixekizumab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1159
Spacegroup name	C 2 2 2
Unit cell lengths	94.445, 127.304, 43.023
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.650 - 1.914
R-factor	0.182
Rwork	0.180
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.9)
Phasing software	PHASER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.652	63.652	2.020
High resolution limit [Å]	1.914	6.050	1.914
Rmerge		0.019	0.480
Rmeas	0.065	0.022	0.592
Rpim	0.026	0.010	0.240
Total number of observations	116086
Number of reflections	19243	715	2122
<I/σ(I)>	20.8	31.7	1.6
Completeness [%]	94.3	98.9	73.7
Redundancy	6	5.5	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	294	2800mM Sodium Acetate pH 7