6NOD

Crystal structure of C. elegans PUF-8 in complex with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-05
Detector	RIGAKU SATURN 92
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	109.182, 189.024, 63.156
Unit cell angles	90.00, 103.61, 90.00

Refinement procedure

Resolution	33.788 - 2.547
R-factor	0.2318
Rwork	0.229
R-free	0.28510
RMSD bond length	0.002
RMSD bond angle	0.450
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.547	2.547
Number of reflections	39843
<I/σ(I)>	9.4
Completeness [%]	98.9	98
Redundancy	6.9	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	3.6 M sodium formate and 10 mM betaine hydrochloride or 3.6 M sodium formate and 3% (w/v) dextran sulfate