6NOC

Crystal structure of FBF-2 repeat 5 mutant (C363A, R364Y) in complex with 8-nt RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-23
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54
Spacegroup name	P 61
Unit cell lengths	95.999, 95.999, 100.428
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.458 - 2.849
R-factor	0.225
Rwork	0.219
R-free	0.27720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k5q
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.849	2.849
Rpim	0.060	0.336
Number of reflections	12319
<I/σ(I)>	12.8
Completeness [%]	99.6	99.2
Redundancy	10.7	8.8
CC(1/2)		0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	100 mM Tris pH 8.0, 12% [w/v] PEG 8000, 8% [v/v] ethylene glycol