6NNY
Crystal Structure of the All-Trans Retinal-Bound R111K:Y134F:T54V:R132Q:P39E:R59Y:L121E Mutant of Human Cellular Retinoic Acid Binding Protein II in the Dark at 1.67 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-02-15 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97872 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 57.962, 57.962, 99.591 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.352 - 1.668 |
R-factor | 0.2119 |
Rwork | 0.209 |
R-free | 0.24850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yfp |
RMSD bond length | 0.006 |
RMSD bond angle | 0.878 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.352 | 1.728 |
High resolution limit [Å] | 1.668 | 1.668 |
Rmerge | 0.066 | 0.779 |
Number of reflections | 23188 | 2269 |
<I/σ(I)> | 37.6 | 2.31 |
Completeness [%] | 99.8 | 99.43 |
Redundancy | 7.2 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 3350, Tacsimate |