6NMV
Non-Blocking Fab 218 anti-SIRP-alpha antibody in complex with SIRP-alpha Variant 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | .97923 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.290, 76.090, 72.810 |
| Unit cell angles | 90.00, 95.26, 90.00 |
Refinement procedure
| Resolution | 38.160 - 2.610 |
| R-factor | 0.2009 |
| Rwork | 0.195 |
| R-free | 0.30070 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.479 |
| Data reduction software | HKL-2000 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.160 | 2.680 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Number of reflections | 45887 | 3382 |
| <I/σ(I)> | 7.5 | 0.7 |
| Completeness [%] | 98.3 | 97.7 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 285 | 0.1 M Tris-HCl, 0.2 M MgCl2, 25% w/v PEG 4000 |
