6NMJ

Crystal Structure of Rat Ric-8A G alpha binding domain, "Paratone-N Immersed"

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	63.298, 100.114, 129.959
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.655 - 2.300
R-factor	0.2094
Rwork	0.208
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nmg
RMSD bond length	0.003
RMSD bond angle	0.570
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.650	2.382
High resolution limit [Å]	2.300	2.300
Rmeas	0.090	1.040
Rpim	0.020	0.200
Number of reflections	37176	3675
<I/σ(I)>	30.72	4.55
Completeness [%]	99.1	99.19
Redundancy	26.3	26.9
CC(1/2)	1.000	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25-30% PEG3350, 0.2 M lithium sulfate, 0.1 M Tris