6NMG

Crystal Structure of Rat Ric-8A G alpha binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-07
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	67.045, 103.645, 141.537
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.050 - 2.200
R-factor	0.2283
Rwork	0.227
R-free	0.27470
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.618
Data reduction software	XDS
Data scaling software	pointless
Phasing software	SHELXD
Refinement software	PHENIX ((1.13rc2_2975: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.050	3.720	2.280
High resolution limit [Å]	2.200	3.410	2.200
Rmeas	0.100	0.370	0.580
Rpim	0.040	0.013	0.280
Number of reflections	50537	1980	4980
<I/σ(I)>	15.4	67.1	2.2
Completeness [%]	99.4	100	99.2
Redundancy	6.4	803.4	4.5
CC(1/2)	1.000	0.980	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25-30% PEG3350, 0.2 M lithium sulfate, 0.1 M Tris
1	VAPOR DIFFUSION, HANGING DROP		293	25-30% PEG3350, 0.2 M lithium sulfate, 0.1 M Tris