6NKE
Wild-type GGGPS from Thermoplasma volcanium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2017-12-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 73.761, 73.761, 122.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.880 - 1.720 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.20410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jej |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.637 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.760 | 1.750 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.071 | |
| Rmeas | 0.075 | |
| Rpim | 0.023 | 0.443 |
| Number of reflections | 36732 | 1916 |
| <I/σ(I)> | 15.2 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.7 | 9.9 |
| CC(1/2) | 0.998 | 0.791 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.04 | 294.15 | 2.2M Ammonium Sulfate, 0.1M Tris pH 8.04 |
