6NKD
Crystal Structure of the Lipase Lip_vut3 from Goat Rumen metagenome.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-26 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 93.120, 93.120, 196.898 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.090 - 2.800 |
| R-factor | 0.2218 |
| Rwork | 0.220 |
| R-free | 0.25650 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.469 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.173 | 0.879 |
| Number of reflections | 13353 | 654 |
| <I/σ(I)> | 32.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 29.2 | 30.6 |
| CC(1/2) | 0.988 | 0.945 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 289 | 2.40 M Ammonium Sulfate, 150 mM Formate pH 4.0, 3.0 % (v/v) 2-Butanol |
